Content of rutin and steroidal saponins extracted from different genotypes from Puncture vine Tribulus terrestris L.

Abstract

In the present study we were evaluated four plant populations of Puncture vine Tribulus terrestris L. from Slovakia (Nové Zámky), Ukraine (Illichivs'k), Portugal (Lisbon) and Turkey (Belek). These plant resources were tested during the years 2012 - 2013 in small plot trials on experimental fields in Presov (the North - Eastern part of Slovakia). The biologically active compounds rutin and steroidal saponins (protodioscin, prototribescin and dioscin) were extracted by 60 % ethanol. The extract contains (HPLC) 1.12-3.95 % of rutin, 0.85-1.85 % of protodioscin, 0.55- 1.15 % of prototribescin and 0.39-0.69 % of dioscin. The highest content (3.95 %) of rutin was found in Slovak and the lowest (1.12 %) in the Portugal genotype.