Морфологічні ефекти застосування інфузії гіперосмолярних розчинів при опіковій травмі шкіри

Abstract

В статті наведені дані щодо структурних змін аденогіпофіза, надниркових залоз, тимуса при експериментальній опіковій травмі шкіри у щурів за умов застосування внутрішньовенної інфузії колоїдно-гіперосмолярних розчинів. Встановлено, що лактопротеїн-С діє як протектор судинної стінки і має мембранопластичний вплив на структуру органів. Ключові слова: опік, аденогіпофіз, надниркова залоза, тимус, світлова та електронна мікроскопія

Experimental study of changes in the adenohypophysis, thymus and adrenal cortex at burn trauma of skin (after 1, 3, 7, 14, 21 and 30 days) were conducted in Wistar rats weight 150-160 g. Maintenance and manipulation of the animals were carried out in accordance with the recommendations " European Convention for the Protection of Vertebrate Animals used for experimental and other scientific purposes". All animals before modeling of pathological condition, the side surface of the body lumps by mechanical machine and safety razor. Burn injury caused by applying 4 copper plates (two plates on each side) that previously held for 6 min. in water at a constant temperature of 100°C. The total area of the burn in rats of indicated weight is 21-23% in exposure 10 sec., which is enough to form the burn of II-III degree and development shock of medium severity. The tentative study showed that rats males without any pharmacological correction on the background of burn injuries of the skin all died on the 9th day of the experiment, and on the seventh day mortality was 80%. That is why all animals carried out corrective infusion with solutions in the inferior vena cava after its aseptically catheterization through the femoral vein. The male Wistar rats were divided into 7 groups. For electron microscope study organs fixed in 2,5% solution of glutaraldehyde in phosphate buffer with 1% OsO4. Dehydration was performed in increasing concentrations of alcohol (70%, 80%, 90%, 100%) and acetone. Impregnated and poured into a mixture of eponaraldite under conventional methods. Ultrathin slices contrasted in 2% solution uranyl acetate and lead citrate. The article presents data in relation to the structural changes in adenohypophysis, adrenal and thymus during experimental burn trauma of skin in rat under the condition of the intravenosis infusion of colloid-hyperosmolar solutions Lactoproteinum-S protects the damage of vessel wall and has a membranoplastic influence on the organic structure. Key words: burn, adenohypophysis, adrenal cortex, thymus, light and electronic microscopy