Перспективи використання Фіто- та антисептичних препаратів для корекції мікробіоти ротової порожнини з урахуванням індивідуальних особливостей асоціацій умовно патогенних мікроорганізмів

Abstract

Abstract. The continuously growing trend for formation and circulation of antibiotic-resistant strains of opportunistic pathogenic microorganisms calls forth the topicality of studies in search of alternative means with antimicrobial properties. It is the representatives of the facultative microbiota of the oral cavity that takes up dominating positions in the structure of inflammatory periodontium. Ander such conditions, constant monitoring of circulating polyresistant strains of opportunistic pathogenic bacteria and elaboration of new approaches to antibacterial therapy acquires a specific significance. The purpose of this paper has been to study the antimicrobial activity of commercial phyto - and antiseptics upon antibiotic-resistant clinical isolates. Object and methods. The isolates that caused periodontium inflammatory processes were isolated on the basis of the Dental Polyclinic, Uzhhorod National University; the antimicrobial activity phyto- and antiseptics was studied at the Microbiological Laboratory of the Department of Genetics, Plant Physiology and Microbiology, Uzhhorod National University; As test culture, the following bacteria and yeast from the American Type Culture Collection were used: Candida albicans ATCC 885-653; Staphylococcus aureus ATCC 25923; Escherichia coli ATCC 25922; Enterococcus faecalis ATCC 29212; Streptococcus pyogenes ATCC 19615; and Pseudomonas aeruginosa ATCC ATCC 27853. We also used clinical strains of bacteria and yeasts (S. aureus, K. rhinoscleromatis, H. alvei, E. coli, S. pyogenes, S. pneumoniae, C. albicans, C. glabrata isolated from the oral cavities of patients suffering from inflammatory periodontium. We chose the clinical strains with multiple resistance to antibiotics. The following domestically produced commercial antiseptics were used in the study: Sangviritrin (DKP Pharmaceutical Factory LLC, Zhytomyr), Tinctura Salviae («Vishpha», Lviv), Chlorophyllipt (Arterium, Halychpharm JSC, Lviv), Stomatofit (Fitofarm, Poland), Decasanum (Yuria-Farm, Kiev, Ukraine), Stomatidin (Bosnalijek d.d.). Results. Among the commercial preparations, it was Sangviritrin that showed the most expressive antimicrobial activity (its antibacterial effect was registered on the clinical isolates of S. aureus). It is worth noting that Sangviritrin showed antimicrobial effect against an extra antibiotic-resistant isolate of K. rhinoscleromatis that was resistant to all the phytopreparations and disnifectants used in the test. A high antimicrobial activity of Decasanum and Stomatidin against multi-antibiotic resistant strains, at most against gram-positive bacteria, was established. Conclusion. Promising is the application of a comprehensive, differential approach to the correction of microbiota in the oral cavity in the conditions of inflammatory process, taking into account the dominant associations and their sensitivity to antibacterial drugs, including phytopreparations, which, in addition to antimicrobial activity, do not violate the composition of indigenous microbiota.