Structural changes of lymph nodes under high calorie diet andmelatonin correction

Abstract

The article presents and analyzes data from an experimental study conducted on whiterats in females and males of reproductive age. The purpose of the study to study themorphometric and histological changes in the parenchyma of the lymph nodes of ratsunder the high-calorie diet (HCD) and with the correction of melatonin. The study wasperformed on 80 white rats of reproductive age. Microanatomy of the structuralcomponents of lymph nodes of white rats under physiological norms was examined in10 intact animals. Experimental animals are divided into 5 groups. Statistical processingof digital data was performed using "Excel" software and "STATISTICA 6.0" using theparametric method. Eight weeks after HCD, there was a significant decrease in therelative area of the cortical substance in the parenchyma of lymph nodes of white ratsof males and females by 10.3 % and 8.3 %, respectively, and an increase in the relativearea of the medullary substance by 16.1 % and 13.2 %, respectively, greater than theintact animal group parameter. Corticomedullary index (CMI) decreased by 22.9 % and19.0 %. After six weeks of HCD and the next six weeks of standard vivarium diet andmelatonin administration, the relative area of cortical substance in the parenchyma oflymph nodes of white rats in males and females was 2.0 % and 2.9 %, respectively,greater than the parameters of the intact group of animals. Accordingly, the relativearea of the medullary substance is 3.1 % and 4.6 % less than the parameters of theintact group of animals. CMI in both male and male rats was 5.1 % and 7.6 %, respectively,greater than the intact animal group parameter. Under the conditions of melatonincorrection, it was found that on the histological preparations of lymph nodes the veinand artery were full-blooded. Empty hemocapillaries with thickened wall are observed.In the paracortical region, the number of high endothelial capillary venules decreases.Thus, long-term administration of melatonin improves the morphometric parameters ofthe parenchyma of the lymph nodes of rats, restores the morphological structure of theorgan