SUBMICROSCOPIC CHANGES OF STRUCTURAL COMPONENTS OF THE SPLEEN DUE TO THE ACTION OF MONOSODIUM GLUTAMATE

Abstract

The results of an experimental study conducted on white male rats and females of reproductive age were analyzed in order to detect submicroscopic changes in the structural components of the spleen under the action of monosodium glutamate in the dynamics. For two, four, six and eight weeks, the animals received monosodium glutamate at a dose of 0.07 g/kg body weight daily with food. Sections of the spleen were made on a UMTP-6M ultramicrotome with a diamond knife (DIATOM) and double contrast was performed according to Reynolds and uranyl acetate. Submicroscopic examinations of the organ were performed using an electron transmission microscope TEM-100. The investigated material was photodocuted using a SONY – H9 digital camera. The first violations of the structural components of the spleen are observed after two weeks, namely the expansion of intercellular spaces in both white and red pulp of the spleen, which contain vacuole-like structures, an increase in plasma cells, the cytoplasm of which is filled with dilated tubules. In dynamics with increase in duration of reception changes deepen, reaching a maximum in 8 weeks of experiment. Electron microscopy revealed that the signs of adaptive-compensatory processes by the end of the experiment lead to a loss of regenerative function.