Effect of the thick extract from garden spinach leaves on bilesecretory and bile-forming function of the liver under conditions of carbon tetrachloride liver lesion

Abstract

The search for new potential drugs with choleretic action has been carried out in recent years among a large number of substances of different structure and origin, but the most promising are drugs of natural, mostly herbal origin. The aim of this research was to study the activity of bile-secreting and bile-forming function of rat liver under conditions of its carbon tetrachloride lesion and after the use of the thick extract from garden spinach leaves. The studies were performed on white male rats kept on the standard diet of the vivarium of Ternopil National Medical University. Animals were divided into 4 groups, three of which were affected by carbon tetrachloride, one served as intact control. One of the groups of affected rats received the cholagogue flamin at the dose of 250 mg/kg body weight, another on the background of carbon tetrachloride liver lesion was exposed to action of thick extract from garden spinach leaves at the dose of 150 mg/kg body weight. It was established that after the lesion of rats with carbon tetrachloride decreases the rate of bile secretion and its volume, which is most pronounced on the 10th day of development of carbon tetrachloride hepatitis. The use of the thick extract from garden spinach leaves led to the restoration of biliary function of the liver, although its effectiveness was somewhat inferior to the reference drug flamin. After the use of both flamin and the studied extract in the serum of rats with carbon tetrachloride liver lesion the content of bile acids and bilirubin decreased during all periods of the experiment (4, 7 and 10 days after exposure to carbon tetrachloride). At the same time the content of bile acids in bile increased. The results of the research make it expedient to further study the thick extract from garden spinach leaves as a cholagogue in order to create on its basis new drugs with hepatoprotective action.