Interrelation between ghrelin and gastrin in patients with combination of chronic gastritis and type 2 diabetes mellitus

Abstract

Introduction: Ghrelin is a 28-amino-acid peptide that is produced by X/A-like cells present in the stomach. Gastrin is a hormone that stimulates gastric acid secretion and mucosal cell growth. The aim is to study the interrelation between ghrelin and gastrin levels in patients with a combination of chronic gastritis and type 2 diabetes mellitus. 60 Helicobacter pylori-positive patients with a combination of chronic gastritis and type 2 diabetes mellitus were examined. The diagnosis of type 2 diabetes mellitus is based on the recommendations of the International Diabetes Federation (IDF, 2005). Gastric acid secretion function was studied by intra-stomach express-pH-meter (method of prof. V.N. Chernobrov). Serum gastrin was determined using ELISA using the Gastrin-EIA test kit Cat. No. CS 001 030. Serum ghrelin was determined by immunoassay analysis using the Human Ghrelin ELISA Kit from RayBiotech No. 1.03930005306. The obtained data testify to the exact between the level of ghrelin and gastrin in the blood of patients with chronic gastritis and type 2 diabetes mellitus. With increasing levels of gastrin in the blood, the level of ghrelin in the blood decreases, and vice versa with a decrease in the level of gastrin in the blood, the level of ghrelin - increases. A significantly higher level of ghrelin was found in patients with type 2 diabetes mellitus and chronic gastritis compared with the control group. The reverse association between gastrin and ghrelin levels in patients with a combination of chronic gastritis and type 2 diabetes mellitus has been obtained.