Impact of polarized low-intense radiation and photosensitizers on growth of Staphylococcus aureus.

Abstract

Due to the growing level of resistance to antimicrobials worldwide, it is important to study the effect of nonmedicamentous means on some biological properties of infectious agents. Among those means physical factors, particularly low intense radiation occupies an important place. The influence of polarized incoherent low-intensity radiation of different wavelengths, photosensitizers (0.1% aqueous solutions of methylene blue, brilliant green, malachite green, methylene green, azure, crystal violet, fuchsin, and chrysoidin) and the combined effect of these factors on the growth intensity of clinical and collection strains of Staphylococcus aureus were studied. The obtained results were compared to control series. It was found that irradiation of the studied microorganisms caused a weak antimicrobial effect on the studied strains, compared to the control; the use of photosensitizers caused a decrease in the growth rate of investigated microorganisms by 16.6–42.5%. The most significant antimicrobial effect showed crystal violet, malachite green, and brilliant green. With the combined effect of photosensitizers and radiation, a decrease in the growth rate of the studied strains was observed – by 22.4–66.2%. Combinations of methylene blue with red spectrum and crystal violet with yellow spectrum showed the most pronounced antimicrobial activity. Obtained data can be used in treatment of inflammatory purulent processes, caused by Staphylococcus aureus